Coding

Part:BBa_K3032017:Design

Designed by: Denis Baronas   Group: iGEM19_Vilnius-Lithuania   (2019-10-13)


Gal4_Q-PAS1 repressor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 577
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Gal4 domain was fused to Q-PAS1 domain. Sequence was codon optimized for E.coli.


Source

Q-PAS1 domain was extracted from https://www.uniprot.org/uniprot/A5YN35. Gal4 domain from https://www.uniprot.org/uniprot/P04386. DNA sequence was codon optimized for E.coli.

References